What happens during in vitro maturation of bovine cumulus-oocyte-complexes?
In vitro maturation (IVM) in bovine involves culturing immature and unexpanded cumulus-oocyte-complexes (COCs) in a laboratory setting to…
What happens during in vitro maturation of bovine cumulus-oocyte-complexes?
Pre IVM
In vitro maturation (IVM) in bovine involves culturing immature and unexpanded cumulus-oocyte-complexes (COCs) in a laboratory setting to allow them to mature and prepare for fertilisation. This process is a crucial step in bovine in vitro embryo production (IVEP). It involves providing the COCs with a carefully controlled environment that mimics the in vivo conditions they would experience during natural maturation in cattle.
Key stages during bovine IVM include:
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Oocyte Collection and Selection: – Immature oocytes are typically collected from ovaries obtained from abattoirs or from live animals. – These oocytes are then sorted and graded based on their morphology and quality
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Maturation Media and Culture Conditions: – Oocytes are placed in a specialised culture medium, often containing a combination of hormones, growth factors, and nutrients that support their maturation. – The culture conditions are set to 38.5°C, 6% CO2 in air for a period of 22–24 hours, during which the oocytes undergo nuclear and cytoplasmic changes necessary for fertilisation.
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Nuclear Maturation: – During IVM, oocytes complete meiosis I and progress to metaphase II (MII), the stage where they are ready for fertilisation. – This nuclear maturation involves the breakdown of the germinal vesicle (GV) and the formation of the first polar body.
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Cytoplasmic Maturation: – Cytoplasmic maturation refers to the changes in the oocyte’s cytoplasm, including the redistribution of organelles, accumulation of maternal mRNA, and acquisition of developmental competence. – These cytoplasmic changes are essential for supporting early embryonic development in the later stages of the in vitro embryo production process.
Read the full article here: https://www.artlabsolutions.com/uncategorized/what-happens-during-in-vitro-maturation-of-bovine-cumulus-oocyte-complexes/
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Post IVM
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