Mouse IgE(Immunoglobulin E) ELISA Kit

Catalog NO.：FY-EM3906

Feiyue’s Mouse IgE is an ELISA reagent for detection of IgE antibody, plasma or cell with sensitivity, specificity and consistency.

Synonyms: IgE ELISA Kit,Immunoglobulin A ELISA Kit

Catalog NO.

FY-EM3906

Reactivity

Mouse

Assay range

3.125–200ng/ml

sensitivity

1. 875ng/ml

Mouse IgE(Immunoglobulin E) ELISA Kit

IgE Introduction：

IgE is a type of antibody (or immunoglobulin (Ig) “isotype”) that has been found only in mammals. IgE is synthesised by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing four Ig-like constant domains (Cε1–Cε4). IgE is thought to be an important part of the immune response against infection by certain parasitic worms, including Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE is also utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. IgE may have evolved as a defense to protect against venoms. IgE also has an essential role in type I hypersensitivity, which manifests in various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergies, and specific types of chronic urticaria and atopic dermatitis.

Mouse IgE(Immunoglobulin E) ELISA Kit Test Method：

This kit uses sandwich ELISA to detect the concentration of Mouse IgE.

Mouse IgE-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse IgE antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Mouse IgE in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

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Reference:

1. “Antibody structure”. Archived from the original on September 6, 2008.

2. Erb KJ (May 2007). “Helminths, allergic disorders and IgE-mediated immune responses: where do we stand?”. European Journal of Immunology. 37 (5): 1170–3. doi:10.1002/eji.200737314. PMID 17447233. S2CID 24519249.

3. Watanabe N, Bruschi F, Korenaga M (April 2005). “IgE: a question of protective immunity in Trichinella spiralis infection”. Trends in Parasitology. 21 (4): 175–8. doi:10.1016/j.pt.2005.02.010. PMID 15780839.