BIO HUM AI SCI JE Jeevitha C M What Is a Variant? SNPs, Indels, and why mutations in the genome actually matter We have spent this week learning how to sequence DNA.
BIO GEN JE Jeevitha C M Before NGS, there was “Sanger” (The sequencing technology that started everything) We have covered a lot of sequencing ground this week.
BIO GEN JE Jeevitha C M Single-Cell RNA-Seq (Why bulk RNA-Seq is not Enough…) We have spent the last few days going deep into RNA-seq, if you haven’t checked, see the previous writeups. The pipeline. The tools. The…
BIO SCI TCH GEN JE Jeevitha C M How to Visualise RNA-Seq results : Volcano Plot, Heatmap and PCA Explained You have run your quality control. You have aligned your reads. You have counted them. You have run DESeq2, and you have a results table…
BIO GEN AI SCI JE Jeevitha C M The tools behind RNA-Seq: HISAT2, DESeq2, and Others Previously, I described the RNA-seq pipeline as moving through four steps: quality control, alignment, quantification, and differential…
BIO HUM GEN MDA TCH JE Jeevitha C M What Is RNA-Seq? A biology student’s guide to read -Gene Expression Data Here is a question I want you to sit with for a moment. Two cells. Same organism. Same DNA. Same genome sequence.
BIO SCI JE Jeevitha C M You got your BLAST results yesterday. Now, what do they mean in terms of biological understanding? Getting results is the easy part. Understanding what they are telling you biologically, that is what the real work is.
BIO AI SCI JE Jeevitha C M Everyone uses BLAST, but do you really use it correctly? My honest opinion on the most fundamental tool in bioinformatics and the three ways people get it completely wrong.
